genetic characterization of fasciola isolates from west azerbaijan province iran based on its1 and its2 sequence of ribosomal dna
نویسندگان
چکیده
abstract background: fascioliasis, caused by fasciola hepatica and f. gigantica , has medical and economic importance in the world. molecular approaches comparing traditional methods using for identification and characterization of fasciola spp. are precise and reliable. the aims of current study were molecular characterization of fasciola spp. in west azerbaijan province, iran and then comparative analysis of them using genbank sequences. methods: a total number of 580 isolates were collected from different hosts in five cities of west azerbaijan province, in 2014 from 90 slaughtered cattle (n=50) and sheep (n=40). after morphological identification and dna extraction, designing specific primer were used to amplification of its1, 5.8s and its2 regions, 50 samples were conducted to sequence, randomly. result: using morphometric characters 99.14% and 0.86% of isolates identified as f. hepatica and f. gigantica, respectively. pcr amplification of 1081 bp fragment and sequencing result showed 100% similarity with f. hepatica in its1 (428 bp), 5.8s (158 bp), and its2 (366 bp) regions. sequence comparison among current study sequences and genbank data showed 98% identity with 11 nucleotide mismatches. however, in phylogenetic tree f. hepatica sequences of west azerbaijan province, iran, were in a close relationship with iranian, asian, and african isolates. conclusions: only f. hepatica species is distributed among sheep and cattle in west azerbaijan province iran. however, 5 and 6 bp variation in its1 and its2 regions, respectively, is not enough to separate of fasciola spp. therefore, more studies are essential for designing new molecular markers to correct species identification.
منابع مشابه
Genetic Characterization of Fasciola Isolates from West Azerbaijan Province Iran Based on ITS1 and ITS2 Sequence of Ribosomal DNA.
BACKGROUND Fascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analys...
متن کاملشناسایی مولکولی گونه‌های فاسیولا در استان آذربایجان غربی
Background & Aims: Fascioliasis caused by Fasciola hepatica and Fasciola gigantica has medical and economic importance in the world. Traditional approaches are not accurate and reliable in identification of agent parasites. Thus the present study was designed to identify the Fasciola sppby molecular methods in West Azerbaijan province.Materials & Methods: In current study Fasciola isolates wer...
متن کاملMolecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses.
This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with α-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as rDNA ma...
متن کاملa study on the design of bio-ethanol process from date wastes of sistan and baluchistan province
اتانول کاربردهای متنوعی در صنایع لاستیک سازی، رنگسازی، حلالها ومکمل سوخت خودرو دارد. اتانول برخلاف نفت از جمله مواد تجدیدپذیر محسوب می شود که مشکلات زیست محیطی و آلودگی نیز ایجاد نمی کند. استفاده از اتانول به عنوان مکمل سوختخودروها از جمله مهمترین مصارف صنعتی این ماده بشمار می رود. با توجه به این موضوع تحقیق و توسعه در زمینه تولید اتانول با درجه خلوص بالا در سطح جهان، و نه تنها در کشور های پیشر...
Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2)
BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of parasitologyجلد ۱۱، شماره ۱، صفحات ۵۲-۶۴
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023